Sequential isolation
The Donor Difference
Counting protocolIsolated cells Isolated
B Cells
CD19+ · positive or negative selection
Adaptive immune cells that produce antibodies, present antigen and secrete immunomodulatory cytokines. Valued for antibody discovery, immune signaling and memory research.
MarkerCD19+Unit10MSourceLeukopak
Formats
Formats, attributes and unit sizes
Fresh and cryopreserved from single-donor leukopaks. Bulk isolation from fresh and cryopreserved full and half leukopaks.
| Product | Cryopreserved |
|---|---|
| CD19+ B, Negative Selection | 10M |
| CD19+ B, Positive Selection | 10M |
Method
Isolation and applications
- Positive immunomagnetic separation. Beads bind the target cells, which are retained while non-target cells are washed away.
- Negative immunomagnetic separation. Beads bind non-target cells, which are depleted, leaving the unlabeled cells of interest.
- Quality control. Cell count and viability checked with a validated acridine orange / propidium iodide (AO/PI) protocol.
Fundamental research (proliferation, biomarker discovery)Drug discoveryImmuno-oncologyCell therapy process development
Learn more
Resources
Protocols, webinars and articles from our scientists. Technical questions go straight to the Customer Success Team.
Bulk
Need more than a vial?
AllCells® Bulk Cell Isolation
Bulk quantities of quality-controlled CD34+ HSPCs, T, B and NK cells, monocytes and PBMCs from single-donor leukapheresis and bone marrow. Bank cells for experimental consistency; custom donor requests and isolation protocols on request.
