Sequential isolation
The Donor Difference
Counting protocolMobilized Leukopak
Collected from healthy donors given FDA-approved drugs that mobilize CD34+ hematopoietic stem and progenitor cells into the blood. Ease of collection, high single-donor CD34+ yield, and the shortest lead time on the market.
Mobilization regimens and formats
Four regimens trade off yield, timing and donor burden. Dual mobilization (Reg H) enhances CD34+ yield across all donor types.
| Product | Regimen | Fresh | Cryopreserved CD34+ |
|---|---|---|---|
| mLP Reg F | G-CSF 10 µg/kg/day × 5 days · apheresis days 5 + 6 | 1 or 2 bags | — |
| mLP Reg H | G-CSF × 5 days + plerixafor 240 µg/kg/day × 2 (evenings of days 4 + 5) · apheresis days 5 + 6 | 1 or 2 bags | — |
| mLP Reg A | Plerixafor 240 µg/kg × 1 day · 11 h injection-to-collection · apheresis day 2 | 1 bag | — |
| mLP Reg B | Plerixafor 240 µg/kg/day × 2 days · 11 h injection-to-collection · apheresis days 2 + 3 | 2 bags | — |
| Reg F CD34+ | G-CSF × 5 days | — | 0.5M1M5M10M25M |
| Reg H CD34+ | Dual mobilization | — | 1M5M10M25M |
| Reg B CD34+ | Plerixafor × 2 days | — | 1M5M10M25M |
Donors receive subcutaneous injections from qualified clinicians.
From collection to isolated HSPCs
Isolated CD34+ cells
CD34+ cells are isolated from any mobilized leukapheresis collection by positive immunomagnetic separation: CD34+ beads bind the target cells and unwanted cells are washed away.
Viable CD34+ counts are quantified by the ISHAGE four-parameter flow cytometry protocol on request.
Made-to-order
Custom donor requests, mobilization regimens, bulk isolations and cryopreservation are available. Everything is made on-site, from donor procurement through shipment.
Resources
Protocols, webinars and articles from our scientists. Technical questions go straight to the Customer Success Team.
