Sequential isolation
The Donor Difference
Counting protocolPBMCs
Isolated from leukopaks and containing T cells (CD3+, CD4+, CD8+), B cells (CD19+), NK cells (CD56+) and monocytes (CD14+). All cryopreserved PBMC inventory is immunophenotyped by flow cytometry, data on request.
Formats, attributes and unit sizes
Fresh and cryopreserved from single-donor leukopaks. Bulk isolation from fresh and cryopreserved full and half leukopaks.
| Tissue source | Product | Cryopreserved | Fresh |
|---|---|---|---|
| Leukopaks | MNC | 10M25M50M100M | 2B4B7B |
PBMCs are isolated from leukopaks treated with ammonium chloride to remove red cells, then washed to remove platelets. PBMCs from whole blood are offered for donor screening. Other PBMC and matched-set products.
Isolation and applications
- Positive immunomagnetic separation. Beads bind the target cells, which are retained while non-target cells are washed away.
- Negative immunomagnetic separation. Beads bind non-target cells, which are depleted, leaving the unlabeled cells of interest.
- Quality control. Cell count and viability checked with a validated acridine orange / propidium iodide (AO/PI) protocol.
Resources
Protocols, webinars and articles from our scientists. Technical questions go straight to the Customer Success Team.
Need more than a vial?
AllCells® Bulk Cell Isolation
Bulk quantities of quality-controlled CD34+ HSPCs, T, B and NK cells, monocytes and PBMCs from single-donor leukapheresis and bone marrow. Bank cells for experimental consistency; custom donor requests and isolation protocols on request.
